Phytochemical profile and antioxidant, antibacterial, anti-inflammatory and anti-sickle cell activities of Vigna radiata (L.) R.Wilczek (Fabaceae) cultivated in DR Congo
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Abstract
Plants, especially medicinal varieties, play a vital role in human health. This study aims to analyze the phytochemical properties and evaluate the antisickling activity of Vigna radiata, cultivated in the Democratic Republic of Congo. Results indicate the presence of various chemical compounds, including total polyphenols (239.84 ± 0.57 mg EQ/g), flavonoids (8.089 ± 0.046 mg EQ/g), anthocyanins (0.503 ± 0.003 mg/g), hydrolyzable tannins (0.084 ± 0.002%), and condensed tannins (0.041 ± 0.001%), along with a foam index of 150 for saponins. The mineral composition, determined through X-ray fluorescence, revealed significant levels of Ca, K, Mg, Fe, Cu, Zn, and Mn. Extraction of V. radiata seeds was performed with solvents of increasing polarity, and the aqueous extract displayed notable antioxidant activity at 25 µg/mL, with an 81.923±8.9% inhibition rate. Furthermore, the DCM extract demonstrated anti-inflammatory effects at 3 mg/mL, inhibiting egg albumin denaturation by 22.72±2.4%. The aqueous extract exhibited antibacterial effects, inhibiting Escherichia coli (18±1.8 mm) and Salmonella SPS (22±3.1 mm), with Staphylococcus aureus showing intermediate sensitivity (10±1.5 mm), whereas Citrobacter diversus showed full resistance. Antisickling activity was significant in the aqueous extract, with normalization rates of 90%, 75%, and 60% at concentrations of 5 µg/mL, 2.5 µg/mL, and 1.25 µg/mL, respectively.
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References
An, Y.J., Kim, M.J., Han, S.I., Chi, H.Y., Kwon, C.,
Kim, S.Y., et al. (2020) Comparison of Chemical Constituents in Mung bean (Vigna radiata L.)
Flour between Cultivation Regions and Seeding Dates. Korean J. Crop Sci. 65(4): 457~467. DOI:
https://doi.org/10.7740/kjcs.2020.65.4.457 .
Bongo, G., Inkoto, C., Masengo, C., Tshiama, C.,
Lengbiye, E., Djolu, R., et al. (2017) Assessment
of Antisickling, Antioxidant and Antibacterial
Activities of Some Congolese Taxa: Aframomum
alboviolaceum (Ridley) K. Schum, Annona
senegalensis Pers. and World Whitei (Hook. f.)
Skeels. American Journal of Laboratory
Medicine. 2(4): 52-59.
Bruneton, J. (1999) Pharmacognosy, Phytochemistry
of Medicinal Plants. 3rd Edition, Revised and
Augmented, Tec & Doc, Paris, 1999.
De Cássia, S.S.R., Luciana, N.A., and Damião, P.D.S.
(2013) “A Review on Anti- Inflammatory
Activity of Monoterpenes ” Molecules 18, no.
:1227-1254.
Dohou, N., Yamni, K., Tahrouch, S., Hassani, L.M.,
Badoc, A.G. (2003) Phytochemical screening of
an Ibero -Moroccan endemic, thymelaea
lythroids. Bull. Soc. Pharma. Bordeaux.
Gbolo, B.Z., Nachtergae, A., Tshibangu, D.S.T.,
Misengabu, N.M., Victoire, N., Memvanga, P.B.,
et al. (2023) In VitroBiological Activities of
Drepanoalpha®Ethanolic extract, a justicia
secunda and moringa oleifera based
phytomedicine proposed for the symptomatic
treatment of sickle cell disease. Journal of
Fundamental and Applied Pharmaceutical
Science.3 (2)
Ikey, A., Kasiama, G.N., Kabengele, C.N., Kapembo,
M.L., Ngoyi, E.M., Bete, J., Kilembe, J.T., et al.
(2022) Phytochemical Profile and Antibacterial
Photodynamic Activity of Centella asiatica L.
(Apiaceae), Citrus grandis L. (Rutaceae) and Zanthoxylum gilletii De Wild. (Rutaceae) Leaves
Excerpts. International Journal of Pathogen Research. 9(2): 1-8.
Kabengele, N.C., Ngoyi, M.E., Kasiama, N.G.,
Kilembe, T.J., Matondo, A., Mpiana, T.P., et al.
(2020) Antihelminthic Activity, Phytochemical
Profile and Microscopic Characteristics of
Ocimum basilicum Collected in DR Congo. Asian
Journal of Biology. 10( 3): 42-50.
Kabengele, C.N., Kasiama, G.N., Ngoyi, E.M.,
Kilembe, J.T., Bete, J., Tshilanda, D.D., et al.
(2022). Secondary Metabolites and Mineral
Elements of Manotes expanded and Aframomum
alboviolaceum Leaves collected in the
Democratic Republic of Congo. Annual Research
& Review in Biology. 37(11): 57-63.
Kambale, J.K., Ngbolua, K.N., Mpiana, P.T., Mudogo,
V., Tshibangu, D.S.T., Wumba, D.M.R., et al.
(2013) In vitro evaluation of the antifalciform
activity and antioxidant effect of Uapaca extracts.
heudelotii Baill. (Euphorbiaceae). 7(2): 523-534.
Kasiama, G.N., Ikey, A., Kabengele, C.N., Kilembe, J.T., Ngoyi, E.M., Bete, J.M., et al. (2022) Anthelmintic and Antioxidant Activities, Phytochemical Profile and Microscopic Characteristics of Senna alata Collected in the Democratic Republic of Congo. Annual Research & Review in Biology. 37(6): 28-36.
Kitadi, J.M., Lengbiye, E.M., Gbolo, B.Z., Inkoto, C.L., Muanyishay, C.L., Lufuluabo, G.L., et al. (2019a) Justice second Vahl species: Phytochemistry, Pharmacology and Future Directions: A mini- review. Discovery Phytomedicine 6(4): 157-171. DOI:10.15562/ phytomedicine.2019.93.
Kitadi, J.M., Inkoto, C.L., Lengbiye, E.M., Tshibangu, D.S.T., Tshilanda, D.D., Ngbolua, K.N., et al. (2019b) Antisickling activity and mineral content of Hura crepitans L., Alternanthera bettsichiana ( rule ) G. Nicholson and Dissotis brazzae cogn , plants used in the management of sickle cell disease in Kwilu province, Democratic Republic of the Congo. European Journal of Pharmaceutical and Medical Research. 6(12): 79-83.
Kumar, V.L and Singhal, A. (2009) Germinating seeds of the mung bean, Vigna radiata (Fabaceae), as a model for the preliminary evaluation of cytotoxic effects of drugs. Biocell. 33(1): 19-24
Lebreton, P., Jay, M., Voirin, B. (1967) On the qualitative and quantitative analysis of flavonoids. Chem. Anal. (Paris). 49(7):375-383.
Matondo, A., Kilembe, J.T., Ngoyi, E.M., Kabengele, C.N., Kasiama, G.N., Mpiana, P.T., et al. (2021) Oleanolic Acid, Ursolic Acid and Apigenin from Ocimum basilicum as Potential Inhibitors of the SARS-CoV-2 Main Protease : A Molecular Docking Study . International Journal of Pathogen Research.6 (2): 1-16.
Mpiana, P.T., Ngbolua, K.N., Tshibangu, D.S.T., Kilembe, J.T., Gbolo, B.Z., Mwanangombo, D.T., et al. (2020) Aloe vera (L.) Burm. F. as a potential anti COVID-19 plant: A minireview of its antiviral activity. Eur J of Med Plants. 31 (8):86-93. doi :10.9734 /EJMP/2020/v31i830261
Mpiana, P.T., Ngbolua, K.N., Bokota, M.T., Kasonga, T.K., Atibu, E.K., Tshibangu, D.S., Mudogo, V. (2010). In vitro effects of anthocyanin extract from Justice second Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfused. 8: 248-254.
Mpiana, P.T., Mudogo, V., Kabangu, Y.F., Tshibangu, D.S.T., Ngbolua, K.N., Atibu, E.K., et al. (2009a). Antisickling activity and thermostability of anthocyanins extract from a congolese plant, Hymenocardia acid Tul. (Hymenocardiaceae). Int. J. Pharmacol.5.65-70.
Mpiana, P.T., Balangayi, E.K., Kanangila, A.B., Kalonda, E.M., Ngbolua, K.N., Tshibangu, D.S.T., et al. (2009b) Antisickle cell activity and thermodegradation of anthocyanins extracted from Sterculia quinqueloba and Ficus capensis. Int. J. Biol.Chem. Sci.3.551-560.
Mpiana, P.T., Ngbolua, K.N., Tshibangu, D.S.T. (2016) Nutraceuticals and sickle cell disease: mini-review, Rendus Chimie report.19.884-889.
Mpiana, P.T., Ngbolua, K.N., and Mudogo, V. (2012) The potential effectiveness of medicinal plants used for the treatment of sickle cell disease in the Democratic Republic of Congo folk medicine: A review. In: Gupta VK, Singh GD (eds), Traditional and Folk Herbal Medicine , Daya Publishing House, New Delhi, India 1:1-11.
Ngbolua, K.N., Mbadiko, M.C., Matondo, A., Bongo, N.G., Ngoyi, E.M., Falanga, C.M., et al. (2020) Review on Ethno-botany, Virucidal Activity, Phytochemistry and Toxicology of Solanum genus: Potential Bio-resources for the Therapeutic Management of Covid-19. Eur J of Nutrition and Food Safety.12 (7):35-48.doi: 10.9734/EJNFS/2020/v12i730246
Ngbolua, K.N., Bishola, T.T., Mpiana, P.T. (2014) In vitro antisickling and free radical scavenging Activities of Pentaclethra macrophylla Benth. (Fabaceae). J Adv Med Life Sci 1:1-6
Ngoyi, E.M., Tshilanda, D.D., Kilembe, J.T., Lengbiye, E.M., Kabengele, C.N., Kasiama, G.N., et al. (2020). Phytochemical profile, antioxidant and anthelmintic activities of O. gratissimum leaves collected in Kinshasa (DR Congo). Discovery Phytomedicine 7(4): 171-176. DOI: 10.15562/phytomedicine.2020.145
Nwar, F.A., Atif, S.L., Rzybylski, R.P., Ultana, B.S., ET, M.A. (2007) Chemical Composition and Antioxidant Activity of Seeds of Different Cultivars of Mung bean, Journal Of Food Science, Vol 72(7).
Ouedraogo, M., Hema, A., Bazié, B.S.R., Zida, S.W.F.M., Batieno, B.J.T., et al. (2021) Identification of major flavonoids using HPLC-MS/MS and determination of antioxidant potential of Vigna radiata seed extracts, Soc. West -Afr. Chim , Vol (050): 57 – 67.
Priya, J.P.A., SudhaLakshmi, Y.G., Banu, F., Gopalakrishnan, S., Dhanalakshmi, P., et al. (2012). Phytochemical screening and antibacterial activity of Vigna radiata L. against bacterial pathogens involved in food spoilage and food borne diseases. J.Acad. Indus. Res. 1(6)
Saini, M., Singh, S., Hussain, Z and Yadav, A. (2010) RAPD analysis in mungbean [Vigna radiata (L.) Wilczek]: I. Assessment of genetic diversity. Indian Journal of Biotechnology. 9:137-146.
Samira, F and Nabil, B. (2020) In vitro study of anti- inflammatory properties of methanolic extract fruits from Rosa canina L.( Rosaceae ), Master thesis, University of Batna, Algeria.
Sguera, S. (2008) Spirulina platensis and its constituents: nutritional interests and therapeutic activities. Pharmaceutical Sciences. hal-01732214.
Shrinivas, S., Dinesh, S., Sagar, D., Tukai, K., Danish, F. (2020) Phytochemical and Pharmacological Evaluation of Vigna radiata Stem Bark Extracts for Its Hepatoprotective Activity. Int journal of pharm and pharmaceuticals. .19 (3).
Tshilanda, D.D., Inkoto, C.L., Mpongu, M., Mata, S., Mutwale, P.K., Tshibangu, D.S.T., et al. (2019). Microscopic Studies, Phytochemical and Biological Screenings of Ocimum canum. International Journal of Pharmacy and Chemistry.5.5.61-67.
Tshilanda, D.D., Onyamboko, D.N.V., Ngbolua, K.N. (2015) Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae). Nat Prod Bio prospect 5: 215-221.
Wagner, H., Bladt, S., Zgainski, E.M. (1984) Plant Drug Analysis; A Thin Layer Chromatography, Atlas, 1984, Germany.