Isolation of bacteria in the aseptic surgery and neonatology rooms of the Ngaliema Clinic and the King Beaudoin I Hospital of Masina in Kinshasa, DRC
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Abstract
Surgery and neonatology rooms must be free of all biotamination. The presence of pathogenic bacteria in the environment of these rooms could lead to infection of patients, resulting in an increase of morbidity and mortality. This study aimed to detect the presence of pathogenic resistant bacteria to antibiotics in the surgery and neonatology rooms of the Ngaliema Clinic and the King Beaudoin I Hospital of Masina. Indoor air of the rooms, the swab of the surfaces of the incubators, operating beds and the handles of the doors as well as the nurses' coats were analyzed. Bacteria were identified by standard bacteriology methods and antibiotic sensitivity was performed by the Müller Hinton agar medium diffusion method. 21 swabs and 34 air samples were collected. 63 strains of bacterial were isolated among them 30 at the Ngaliema clinic and 33 at the King Beaudoin I Hospital. The isolated bacteria were Staphylococcus aureus (34.9%), Staphylococcus spp (30.2%), Bacillus spp (12.7%), Klebsiella pneumoniae (9.5%), Pseudomonas spp (7.90%), Klebsiella ozaenae (1.6%), Providencia alcalifaciens (1.6%) and Acinetobacter spp (1.6%). 52.2% of strains were multi-resistant to the majority of antibiotics tested. The results of the present study showed that multi-resistant bacteria are circulating in the neonatology and surgery rooms of these hospitals. Infection prevention and control measures should be considered.
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