Ciprofloxacin radiolabeling with technetium-99m
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Abstract
In the Democratic Republic of Congo and many developing nations, undiagnosed or inadequately diagnosed bacterial infections continue to be a significant contributor to mortality rates. Nuclear medicine employs rapid and precise techniques utilizing radiopharmaceutical kits, which are essential for the diagnosis of deep infections. The main goal of this study was to make technetium-99m (99mTc)-labeled ciprofloxacin and test its stability. The department of nuclear medicine at Kinshasa University Clinics planned to use this kit to diagnose infection foci. Ciprofloxacin is radiolabeled by combining 20 mCi of 99mTc with a solution of ciprofloxacin (CPF) at a concentration of 15 mg/mL, 200 mg of ascorbic acid, and 10 mL (0.2 mg/mL) of freshly prepared stannous chloride (SnCl.2HO) in a 2N hydrochloric acid solution at pH 5.8.
Thin layer chromatography (TLC) was employed alongside the radiochromatogram to assess radiochemical purity and stability at room temperature. The radiochemical purity of the product exceeded 98%, and its stability after 4 hours was 95%. The preparation method for 99mTc-CPF is straightforward and demonstrates high labeling efficiency. The 99mTc-CPF preparation method may function as an effective radiopharmaceutical kit for imaging infectious foci.
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